Unit Description

This unit is designed for the development of new knowledge, products, and services concerning genomics, molecular biology, proteomics, and cellular biology implementing innovative technologies in an ethical and amicable environment of the highest quality for health, pharmaceutical and agro-food industries, aiming to be a leader on development based in nucleic acids and protein research.

 

This unit has Next-Generation Sequencing (NGS) platforms, nucleic acid microarray, Proteomics, real time PCR and cell culture. In addition, this unit has the appropriate facilities to perform molecular diagnosis in health, agronomy, veterinary, and biotechnology areas. The unit can offer and provide training to other research centers and/or researchers in genomic tools appliance.

In recent years, Genomics Unit has implemented an ensemble of research on preclinic methods (cell and mice) to help valuate DL50, toxicity, and the functional effects of certain molecules with clinical appliance.

Unit Chief

Q.C.B Patricia Villarreal Quiroga

E-mail: patricia.villarrealq@uanl.mx

Phone Number: +52(81) 1340 4370 ext 1724, 1718 or 1716

Microarray

  • Genotyping (SNP’s, CNV’s)
  • Gene expression
  • MicroRNA’s
  • Drug metabolism (DMET)
  • Molecular cytogenetic

Next-Generation Sequencing

  • Smaller genome sequencing
  • Transcriptome
  • Metagenomics
  • Pharmacogenomics
  • Gene paneling associated to cancer
  • Personalized gene paneling

PCR and real time PCR (qPCR)

  • Absolute quantification
  • Relative quantification
  • Allelic discrimination
  • CNV’s analysis

Cell culture

  • Maintenance and growth of diverse cell lines
  • Essays on apoptosis, differentiation, invasion, migration, and adipogenesis
  • Essays on 3D culture
  • Essays on in vitro (cell lines) and in vivo (murine model) new molecule models
  • Essays on feasibility and toxicity in models of hypoxia and acidity

Molecular diagnosis

  • Detection of pathogens (bacteria and viruses)
  • Detection of genetic variation and mutations (SNP’s)
  • Molecular cytogenetic
  • FISH for various targets

Proteomics

  • Two-dimensional electrophoresis
  • Protein extraction and quantification
  • Western blot
  • Protein identification by mass Spectrometry
  • SDS-PAGE

Microarrays

  • Affymetrix Microarrays Platform
  • NimbleGen Microarrays Platform

Sequencing

  • MiSeq illumine system

 

PCR

  • GeneAmp 9700
  • Veriti 9902
  • Tetrad 2
  • MasterCycler
  • GeneAmp 2400

Real time PCR

  • Light Cycler 480 II

Proteomics

  • GelDoc XR
  • ChemiDoc XRS
  • Exquest/spot cutter

Cell Culture

  • CO2 Incubator
  • Class II A2 Biosafety Cabinet
  • Microscope
  • Inverted Microscope
  • Fluorescent Microscope
  • Plaque luminometer
  • Hypoxia camera
  • Cryogenic tank

Others

  • Experion
  • Bioanalyzer 2100
  • NanoDrop 8000
  • Spectrophotometer Smart Plus
  • Orbital thermo-shaker
  • Sent: E1. BMC Cancer / # BCAN-D-15-00838 Gene expression signature of TNBC vs nTNBC highlights biomarkers involved in cell metabolism Sandra Karina Santuario-Facio; Servando Cardona-Huerta; Yadira Xitlalli Perez-Paramo; Victor Trevino; Francisco Hernandez-Cabrera; Augusto Rojas-Martinez; Lizeth Martinez-Jacobo; Gerardo Padilla-Rivas; Gerardo Muñoz-Maldonado; Jorge Luis Martinez-Rodriguez; Juan F. Gonzalez-Guerrero; Javier Valero-Gomez; Ana L. Vazquez-Guerrero; Herminia Guadalupe Martinez-Rodriguez; Alvaro Barboza-Quintana; Raquel Garza-Guajardo; Oralia Barboza-Quintana; Rocio Ortiz-Lopez, Ph. D. (corresponding)E2.  Ciencia UANL. Adriana Sánchez-García, Viviana C. Zomosa-Signoret, RocíoOrtiz-López and Román Vidaltamayo. Orexina y sus Aplicaciones en la Clínica.E3: Frontiers in Molecular Neuroscience Adriana Sánchez-García*, Rocío Ortiz-López*,**, Viviana Chantal Zomosa-Signoret*, Román Vidaltamayo***The Transcription Factor ebf2 Activates Gene Expression Driven by a Hypocretin Minimal Promoter Region (Ebf2 increases orexin-promoter driven expression)
  • Recent Publishing
    1. Sevilla González FK, Welsh Lozano O, Ortiz López R, Vera Cabrera L, Ocampo Candiani J, Zamora Avila DE, Ocampo Garza J, Hernández Escareño JJ. “Distribution of Malassezia species in Mexican seborrheic dermatitis patients” ACEPTADO. African Journal of Microbiology Research 2016. ISSN 1996-0808.
    1. Mauricio A. Salinas-Santander1, Elizabeth Bazan-Mendoza2, Marisol Espinosa-Ruiz2, Rocio Ortiz-Lopez3,4, Adriana Bustamante5, Celia Sanchez-Domínguez. The +1858 C/T polymorphism in the PTPN22 gene is associated to Cystic Fibrosis patients in Northeast Mexico. Running title: PTPN22 polymorphism in Mexican patients with CF. ACEPTADO. Archives of Medical Research / # ARCMED-D-16-00145
    1. Antonio Ali Perez-Maya, Rosa Maria Hinojosa-Robles, Jose Ramon Barcenas-Walls, Armando Vignau-Cantu, Hugo A. Barrera-Saldaña, Rocio Ortiz-Lopez. Complete Genome Sequence of Streptococcus pneumoniae Serotype 19A, a Blood Clinical Isolate from Northeast Mexico. ACEPTADO. Genome Announcement March/April 2016 4(2) e00195-16
    1. Cuervo-Sierra J, Jaime-Pérez JC, Martínez-Hernández RA, García-Sepúlveda RD, Sánchez-Cárdenas M, Gómez-Almaguer D, Ortíz-López R, Villarreal-Villarreal CD, Ruiz-Arguelles GJ, Ruiz-Delgado G, LutzPresno J, Garcés-Eisele J, Ignacio-Ibarra G, Muciño-Hernández G, Arana-Trejo RM, Jiménez-Mejia AM, Vásquez-Palacio G. Prevalence and Clinical Significance of FLT3 Mutation Status in Acute Myeloid Leukemia Patients: A Multicenter Study. Arch Med Res. 2016. 47(3):172-9.
    2. Gonzalez-Carrillo C, Millan-Sauceda C, Lozano-Garza HG, Ortiz-Lopez R, Elizondo-Gonzalez R, Welsh O, Ocampo-Candiani J, Vera-Cabrera L. Genomic Changes Associated with the Loss of Nocardia brasiliensis Virulence in Mice after 200 In Vitro Passages. Infect Immun. 2016. 19;84(9):2595-606.

QCB. Patricia Villarreal Quiroga

QBP. Laura Rivera Narváez

M.Sc. Luis Ernesto Osuna Rosales

TSU. Carlos Alberto Hernández Puente

Juanita González Domínguez

 

M.Sc. Lizeth Alejandra Martínez Jacobo.

  • Chemical Biologist, Medicine School, UANL
  • Master’s in sciences with orientation in Molecular Biology and genetic engineering, Medicine School, UANL
  • Candidate to PhD in Sciences with orientation in Molecular Biology and Genetic engineering, Medicine School, UANL
  • Certificate in Medical Genetics by the Universitat de Valencia, Valencia, Spain
  • Member of the Mexican School for the Study and Treatment of Congenital Defects

Research Line

  • Multiethnic analysis of androgenetic alopecia
  • Genomic identification on patients with congenital defects and retardation on intellectual development/incapacity of unknown etiology

Projects

  • Genomic identification on patients with congenital defects and retardation on intellectual development/incapacity of unknown etiology.
  • “Correlation of expression GPR44, PTGDS, and PGE2 on total and free testosterone levels on peripheral blood in male and female patients with androgenetic alopecia”. M.C.P. César Daniel Villarreal.
  • “Caspases expression and other apoptosis genes and its relation with testosterone and DHT levels in peripheral blood in male patients with androgenetic alopecia” M.C.P. Ana Cecilia Lamadrid Zertuche.
  • “The effect of dietary supplementation with flavonoids during pregnancy and lactation in Wistar mother rats and its role of obesity in offspring” L.N. Carolina Treviño De Alba.

 

IBT. Bianka Dianey Camacho Zamora

  • Biotechnology Engineer, Polytechnic University of Sinaloa
  • Candidate to Master’s in Science with orientation in Molecular Biology and Genetic engineering, Medical School, UANL

Research Area

  • Analysis of the role of FOXC1 in Epithelial-Mesenchymal Transition in breast cancer
  • Proteomic characterization of breast cancer molecular subtypes

Research projects

  • “Role of the transcription factor of FOXC1 in Epithelial-Mesenchymal Transition in breast cancer”.
  • “Identification of the possible white genes of FOXC1 in cell lines of breast cancer”. Ana Victoria Camero Maldonado.
  • In vitro characterization of protean profiles on MCF7 and MCF12A cell lines”. José Melissa López Pérez.
  • “2D-PAGE technique usage for the comparative proteomic analysis between BT474 and HCC1954 breast cancer cell lines”. Norma Paola Guízar Amador.

 

Undergraduate Students (Consulting)

  • José Mauricio Ayala Esparza, bachelor’s Degree in Genomic Biotechnology
  • Norma Paola Guízar Amador, bachelor’s Degree in Genomic Biotechnology
  • Tania Melissa López Pérez, Q.B.P.

 

Services

Microarray

  • Genotyping (SNP’s, CNV’s)
  • Gene expression
  • MicroRNA’s
  • Drug metabolism (DMET)
  • Molecular cytogenetic

Next-Generation Sequencing

  • Smaller genome sequencing
  • Transcriptome
  • Metagenomics
  • Pharmacogenomics
  • Gene paneling associated to cancer
  • Personalized gene paneling

PCR and real time PCR (qPCR)

  • Absolute quantification
  • Relative quantification
  • Allelic discrimination
  • CNV’s analysis

Cell culture

  • Maintenance and growth of diverse cell lines
  • Essays on apoptosis, differentiation, invasion, migration, and adipogenesis
  • Essays on 3D culture
  • Essays on in vitro (cell lines) and in vivo (murine model) new molecule models
  • Essays on feasibility and toxicity in models of hypoxia and acidity

Molecular diagnosis

  • Detection of pathogens (bacteria and viruses)
  • Detection of genetic variation and mutations (SNP’s)
  • Molecular cytogenetic
  • FISH for various targets

Proteomics

  • Two-dimensional electrophoresis
  • Protein extraction and quantification
  • Western blot
  • Protein identification by mass Spectrometry
  • SDS-PAGE
Facilities and Equipment

Microarrays

  • Affymetrix Microarrays Platform
  • NimbleGen Microarrays Platform

Sequencing

  • MiSeq illumine system

 

PCR

  • GeneAmp 9700
  • Veriti 9902
  • Tetrad 2
  • MasterCycler
  • GeneAmp 2400

Real time PCR

  • Light Cycler 480 II

Proteomics

  • GelDoc XR
  • ChemiDoc XRS
  • Exquest/spot cutter

Cell Culture

  • CO2 Incubator
  • Class II A2 Biosafety Cabinet
  • Microscope
  • Inverted Microscope
  • Fluorescent Microscope
  • Plaque luminometer
  • Hypoxia camera
  • Cryogenic tank

Others

  • Experion
  • Bioanalyzer 2100
  • NanoDrop 8000
  • Spectrophotometer Smart Plus
  • Orbital thermo-shaker
Publishing
  • Sent: E1. BMC Cancer / # BCAN-D-15-00838 Gene expression signature of TNBC vs nTNBC highlights biomarkers involved in cell metabolism Sandra Karina Santuario-Facio; Servando Cardona-Huerta; Yadira Xitlalli Perez-Paramo; Victor Trevino; Francisco Hernandez-Cabrera; Augusto Rojas-Martinez; Lizeth Martinez-Jacobo; Gerardo Padilla-Rivas; Gerardo Muñoz-Maldonado; Jorge Luis Martinez-Rodriguez; Juan F. Gonzalez-Guerrero; Javier Valero-Gomez; Ana L. Vazquez-Guerrero; Herminia Guadalupe Martinez-Rodriguez; Alvaro Barboza-Quintana; Raquel Garza-Guajardo; Oralia Barboza-Quintana; Rocio Ortiz-Lopez, Ph. D. (corresponding)E2.  Ciencia UANL. Adriana Sánchez-García, Viviana C. Zomosa-Signoret, RocíoOrtiz-López and Román Vidaltamayo. Orexina y sus Aplicaciones en la Clínica.E3: Frontiers in Molecular Neuroscience Adriana Sánchez-García*, Rocío Ortiz-López*,**, Viviana Chantal Zomosa-Signoret*, Román Vidaltamayo***The Transcription Factor ebf2 Activates Gene Expression Driven by a Hypocretin Minimal Promoter Region (Ebf2 increases orexin-promoter driven expression)
  • Recent Publishing
    1. Sevilla González FK, Welsh Lozano O, Ortiz López R, Vera Cabrera L, Ocampo Candiani J, Zamora Avila DE, Ocampo Garza J, Hernández Escareño JJ. “Distribution of Malassezia species in Mexican seborrheic dermatitis patients” ACEPTADO. African Journal of Microbiology Research 2016. ISSN 1996-0808.
    1. Mauricio A. Salinas-Santander1, Elizabeth Bazan-Mendoza2, Marisol Espinosa-Ruiz2, Rocio Ortiz-Lopez3,4, Adriana Bustamante5, Celia Sanchez-Domínguez. The +1858 C/T polymorphism in the PTPN22 gene is associated to Cystic Fibrosis patients in Northeast Mexico. Running title: PTPN22 polymorphism in Mexican patients with CF. ACEPTADO. Archives of Medical Research / # ARCMED-D-16-00145
    1. Antonio Ali Perez-Maya, Rosa Maria Hinojosa-Robles, Jose Ramon Barcenas-Walls, Armando Vignau-Cantu, Hugo A. Barrera-Saldaña, Rocio Ortiz-Lopez. Complete Genome Sequence of Streptococcus pneumoniae Serotype 19A, a Blood Clinical Isolate from Northeast Mexico. ACEPTADO. Genome Announcement March/April 2016 4(2) e00195-16
    1. Cuervo-Sierra J, Jaime-Pérez JC, Martínez-Hernández RA, García-Sepúlveda RD, Sánchez-Cárdenas M, Gómez-Almaguer D, Ortíz-López R, Villarreal-Villarreal CD, Ruiz-Arguelles GJ, Ruiz-Delgado G, LutzPresno J, Garcés-Eisele J, Ignacio-Ibarra G, Muciño-Hernández G, Arana-Trejo RM, Jiménez-Mejia AM, Vásquez-Palacio G. Prevalence and Clinical Significance of FLT3 Mutation Status in Acute Myeloid Leukemia Patients: A Multicenter Study. Arch Med Res. 2016. 47(3):172-9.
    2. Gonzalez-Carrillo C, Millan-Sauceda C, Lozano-Garza HG, Ortiz-Lopez R, Elizondo-Gonzalez R, Welsh O, Ocampo-Candiani J, Vera-Cabrera L. Genomic Changes Associated with the Loss of Nocardia brasiliensis Virulence in Mice after 200 In Vitro Passages. Infect Immun. 2016. 19;84(9):2595-606.
Unit Personnel

QCB. Patricia Villarreal Quiroga

QBP. Laura Rivera Narváez

M.Sc. Luis Ernesto Osuna Rosales

TSU. Carlos Alberto Hernández Puente

Juanita González Domínguez

 

M.Sc. Lizeth Alejandra Martínez Jacobo.

  • Chemical Biologist, Medicine School, UANL
  • Master’s in sciences with orientation in Molecular Biology and genetic engineering, Medicine School, UANL
  • Candidate to PhD in Sciences with orientation in Molecular Biology and Genetic engineering, Medicine School, UANL
  • Certificate in Medical Genetics by the Universitat de Valencia, Valencia, Spain
  • Member of the Mexican School for the Study and Treatment of Congenital Defects

Research Line

  • Multiethnic analysis of androgenetic alopecia
  • Genomic identification on patients with congenital defects and retardation on intellectual development/incapacity of unknown etiology

Projects

  • Genomic identification on patients with congenital defects and retardation on intellectual development/incapacity of unknown etiology.
  • “Correlation of expression GPR44, PTGDS, and PGE2 on total and free testosterone levels on peripheral blood in male and female patients with androgenetic alopecia”. M.C.P. César Daniel Villarreal.
  • “Caspases expression and other apoptosis genes and its relation with testosterone and DHT levels in peripheral blood in male patients with androgenetic alopecia” M.C.P. Ana Cecilia Lamadrid Zertuche.
  • “The effect of dietary supplementation with flavonoids during pregnancy and lactation in Wistar mother rats and its role of obesity in offspring” L.N. Carolina Treviño De Alba.

 

IBT. Bianka Dianey Camacho Zamora

  • Biotechnology Engineer, Polytechnic University of Sinaloa
  • Candidate to Master’s in Science with orientation in Molecular Biology and Genetic engineering, Medical School, UANL

Research Area

  • Analysis of the role of FOXC1 in Epithelial-Mesenchymal Transition in breast cancer
  • Proteomic characterization of breast cancer molecular subtypes

Research projects

  • “Role of the transcription factor of FOXC1 in Epithelial-Mesenchymal Transition in breast cancer”.
  • “Identification of the possible white genes of FOXC1 in cell lines of breast cancer”. Ana Victoria Camero Maldonado.
  • In vitro characterization of protean profiles on MCF7 and MCF12A cell lines”. José Melissa López Pérez.
  • “2D-PAGE technique usage for the comparative proteomic analysis between BT474 and HCC1954 breast cancer cell lines”. Norma Paola Guízar Amador.

 

Undergraduate Students (Consulting)

  • José Mauricio Ayala Esparza, bachelor’s Degree in Genomic Biotechnology
  • Norma Paola Guízar Amador, bachelor’s Degree in Genomic Biotechnology
  • Tania Melissa López Pérez, Q.B.P.