Unit Chief

MD Carlos Eduardo Medina de la Garza

E-mail: carlos.medina@uanl.mx

Phone Number: +52(81) 1340 4370 ext 1708 or 1730

Unit Description

An integral part of the Research and Development Center on Health Sciences (CIDICS), the Immunomodulators Unit recognizes immunomodulation as a specific research area to fulfill substantial contributions.

The laboratory is set to perform permanent evaluation of new or already know molecules that can perform as modifiers of immunologic response in medical therapy. Some of the objectives, among others, are to study anthelmintic and antiparasitic drugs with putative immunomodulatory properties such as levamisole, albendazole, ivermectin and diethylcarbamazine.

Diethylcarbamazine (DEC) is an anthelmintic derived from piperazine that has shown immunostimulatory properties including an increase in the adherence of granulocytes and plaques to the parasite. In this laboratory, modulatory properties of DEC have been analyzed regarding the immune and humoral cellular response, as well as the respiratory burst on a BALB/c mouse. Results suggest a stimulatory dosage-dependent effect of DEC, thus, it can be considered as a potential candidate for its usage as an immunologic modifier.

Methodology used for the evaluation processes for this kind of drugs is the measurement of respiratory outburst, cytokines expression, antibody production evaluation and evaluation in animals, amongst others. The services of this laboratory are sustained with Undergraduate and Postgraduate researchers/professors from the Medicine School and a Chemist specialized in basic research and highly qualified in operating the methodologies used for the evaluation of the immune system.

One of the therapeutic purposes of immunomodulation is the remediation of immunodeficiency conditions, mainly secondary immunodeficiency. Secondary immunodeficiencies are present on individuals with pathologies that affect one or more elements of the immune system; meaning that immunologic deficit is a secondary manifestation of an underlying disease (hereditary diseases, nutritional deficiencies, immunosuppressant agents, amongst others).

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3D tissue culture biomimetize the morphologic and functional characteristics of an in vivo parental tissue. The development of an in vitro model of a swollen intestine based on human tissue can be a promising alternative to compliment the experimental systems existent for the monitoring and study of new therapeutic agents; these could help to determine the potential use of immunomodulatory substances against chronic inflammatory and autoimmune diseases.

This unit’s objective is the new therapeutic approach that function with known therapies to counteract diseases. The stimuli of the immunologic effectors in the host, different to the direct microbicide action of antibiotics, antivirals, and antifungal, represents an option to combat chronic pyogenic diseases.

  • The permanent evaluation of new or already know molecules that can perform as modifiers of immunologic response in medical therapy.

Research areas:

  • Molecule evaluation with potential immunomodulators and its use in therapeutic medicine.

Specific projects:

  • “Evaluation of the immunomodulatory effect in the treatment with diethylcarbamazine in a model of dermic injury in diabetic mice”.
  • Development and characterization of an in vitro cell culture 3D model of an intestinal epithelium

Collaborations:

  • Tropical Medicine Institute, Bernhard-Nocht, Germany.
  • Veterinary Medicine School, UANL.
  • Biological Sciences School, UANL.
  • Medicine School, UANL.

This unit has the characteristics of a Level II biosafety laboratory. Basic equipment of a research laboratory such as:

  • Refrigerated centrifugation equipment
  • Refrigeration and deep-freezing equipment
  • Basic equipment for the analysis of proteins

As well as specialized technologies:

  • BD FACSCANTO II flow cytometer
  • AXIOVERT D1 Microscopy with fluorescent microscopy configuration
  • Del Angel-Mosqueda C, Gutiérrez-Puente Y, López-Lozano AP, Romero-Zavaleta RE, Mendiola-Jiménez A, Medina-De la Garza CE, Márquez-M M, De la Garza-Ramos MA. Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro. Head Face Med. 2015 Sep 3;11:29. doi: 10.1186/s13005-015-0086-5.
  • Janssen N, Fernandez-Salas I, Díaz González EE, Gaytan-Burns A, Medina-de la Garza CE, Sanchez-Casas RM, Börstler J, Cadar D, Schmidt-Chanasit J, Jöst H. Mammalophilic feeding behaviour of Culex quinquefasciatus mosquitoes collected in the cities of Chetumal and Cancun, Yucatán Peninsula, Mexico. Trop Med Int Health. 2015 Nov;20(11):1488-1491. doi: 10.1111/tmi.12587.
  • Baca-Castañón ML, De la Garza-Ramos MA, Alcázar-Pizaña AG, Grondin Y, Coronado-Mendoza A, Sánchez-Najera RI, Cárdenas-Estrada E, Medina-De la Garza CE, Escamilla-García E. Antimicrobial Effect of Lactobacillus reuteri on Cariogenic Bacteria Streptococcus gordonii, Streptococcus mutans, and Periodontal Diseases Actinomyces naeslundii and Tannerella forsythia. Probiotics Antimicrob Proteins. 2015 Mar;7(1):1-8. doi: 10.1007/s12602-014-9178-y.
  • Gutiérrez-Gómez C, Godínez-Hana AL, García-Hernández M, Suárez-Roa MD, Toussaint-Caire S, Vega-Memije E, Gutiérrez-Mendoza D, Pérez-Dosal M, Medina-De la Garza CE.Lack of IgG antibody seropositivity to Borrelia burgdorferi in patients with Parry-Romberg syndrome and linear morphea en coup de sabre in Mexico. Int J Dermatol. 2014:DOI:10.1111/ijd.12105
  • Medina-De la Garza Carlos E., García-Hernández Marisela and Castro Corona María de los Ángeles. Visual mnemonics for serum protein electrophoresis. Med Educ Online. 2013; 18: 10.3402/meo.v18i0.22585.
  • Medina De la Garza C. E. El papel de la academia en la ciencia y tecnología. Ingenierias. 2012;(54):3-6
  • Medina-De la Garza CE, Guerrero-Ramírez G, García-Hernández M, Castro-Corona MA, Torres-López E, Brattig NW and Salinas-Carmona MC. Immunomodulatory activity of diethylcarbamazine on humoral, cellular response and respiratory burst in BALB/c mice. Immunopharmacol Immunotoxicol 2012;(34):477-83.
  • Medina De La Garza CE. Parasitosis. En Infectología Clínica. 2a Ed.  Manual Moderno, 2012. pp 363-400
  • Medina De la Garza C. E., Castro Corona M. A. Inmunodeficiencias Secundarias. En Fundamentos para el Ejercicio de la Medicina. 1a Ed. Gúzman López S. y Cedillo Salazar F.E. Manual Moderno, 2012. pp 292-294.
  • Castro Corona M. A., Medina De la Garza C. E. Inmunodeficiencias Primarias. En Fundamentos para el Ejercicio de la Medicina. 1a Ed. Gúzman López S. y Cedillo Salazar F.E. Manual Moderno, 2012. pp 285-291.
  • Medina De la Garza C. E. Técnicas inmunológicas para el estudio de antígenos parasitarios.  En Parasitología Médica. 3a Ed. Becerril Flores MA.  McGraw Hill,   2011. pp 345-351
  • García-Hernández M, Castro-Corona MA, Medina-De la Garza CE. La Inmunomodulación en la terapéutica moderna. Ciencia Conocimiento 2011; 116, mayo 2011. www.conocimientoenlinea.com
  • Leal Villarreal L, Huerta López JG, Salinas Carmona MC, Cárdenas Estrada E, Muñoz Espinosa LE, Castro Corona MA, Medina de la Garza CE. Inmunoterapia sublingual a dosis bajas en pacientes con rinitis alérgica en un estudio doble ciego controlado con placebo. Medicina Universitaria. 2010; 12(46): 3-10
  • Castro Corona M. A., Medina De la Garza C. E. Inmunodeficiencia primarias y secundarias: características, diagnóstico y tratamiento inmunológico. En La Inmunología en la Salud y la Enfermedad. 1a Ed. Salinas Carmona M. C. (ed) Editorial Médica Panamericana, 2010. pp 165-181.
  • Medina De la Garza C. E., Castro Corona M. A. Respuesta Inmune contra infecciones por parásitos: Factores del parásito y del hospedero involucrados en la respuesta a la infección. En La Inmunología en la Salud y la Enfermedad. 1a Ed. Salinas Carmona M. C. (ed) Editorial Médica Panamericana, 2010. pp 283-296.
  • Medina De la Garza C. E., Castro Corona M. A. Salinas Carmona M. C. Alergia y Parásitos. En Alergia enfermedad multisistémica, 1a Ed. Méndez de Inocencio J. et. al. (eds) Editorial Médica Panamericana, 2009. pp 313-320.
  • García-Hernández M, Guerrero-Ramírez G, Castro-Corona MA, Medina-De la Garza CE, Immunomodulators as adjuvant therapy in infection diseases, Medicina Universitaria. 2009;11(45):247-259.